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- DNA Extraction
- DNA Isolation
- DNA Library Preparation
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- Electrophoresis
- FISH - Fluorescent In Situ Hybridization
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- Gel Electrophoresis
- NGS - Next Generation Sequencing
- Optical Microscopy
- Particle Analysis
- PCR - Polymerase Chain Reaction
- qPCR - Quantitative …rase Chain Reaction
- Real-time PCR - Real…rase Chain Reaction
- TOC - Total Organic Carbon Analysis
GFZ Helmholtz Centre for GeosciencesSeveral analysis tools are available for cell-based research e.g. facilities to carry out polymerase chain reactions (PCR). The qTower³ G touch qPCR machine is a 96-well format and currently uses one SYBR Green module, however, can be equipped with up to six for multiplexing capabilities. One main function of this system will be to evaluate stress parameters on microbial communities collected from Arctic snow and ice samples.
GFZ Helmholtz Centre for GeosciencesThe Molecular Microbiology Lab focuses on
I) DNA and RNA extraction, quantification and integrity applying protocols on commercial kits (physical and chemical cell lysis, silica column), phenol/chloroform extractions and extra- and intracellular separation (e/I DNA), and
II) microbiome diversity and abundance applying protocols on amplification and quantification of target genes via PCR and quantitative PCR, NGS library preparation as well as NGS sequencing.
Various sample types such …