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- CRDS - Cavity Ring Down Spectroscopy
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- SPE - Solid-Phase Extraction
- TOC - Total Organic Carbon Analysis
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- Ultrasonic Cleaning Device
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GFZ Helmholtz Centre for GeosciencesThe Culture Lab is equipped with two low-temperature incubators (Percival LT-36VL and E-36VL) and two refrigerators to grow various types of algae under different light conditions and temperatures, including UV-A and UV-B.
Cultures can also be grown on an orbital shaker that can be placed inside the incubator.
Both the LT-36VL and the E-36VL have a white light intensity of up to 1,450 µmol/m-2/s-1, a UV-A light intensity of up to 40 W/m², and a UV-B light intensity of up to 1 W/m². All …
GFZ Helmholtz Centre for GeosciencesSeveral analysis tools are available for cell-based research e.g. facilities to carry out polymerase chain reactions (PCR). The qTower³ G touch qPCR machine is a 96-well format and currently uses one SYBR Green module, however, can be equipped with up to six for multiplexing capabilities. One main function of this system will be to evaluate stress parameters on microbial communities collected from Arctic snow and ice samples.
GFZ Helmholtz Centre for GeosciencesOur Microbiology Laboratories are used for the enrichment, isolation, and characterization of novel microorganisms, including both aerobic and anaerobic species. We conduct phylogenetic and physiological analyses to describe new strains and assess their metabolic potential. Additionally, we perform physiological growth experiments under diverse environmental conditions, including extreme stress factors. Our research includes studies on microbial adaptation to desert environments (hot and …
GFZ Helmholtz Centre for GeosciencesThis lab is dedicated for carrying out various microbial techniques in a sterile environment. These include the isolation of fungi on agar media from soil samples, the purification of DNA from microbial cultures and soil samples, and setting up of soil microcosm experiments. Sterile conditions are created by the laminar flow workbench, the use of sterile disposable instruments (e.g., inoculation loops, pipette tips) and by sterilizing surfaces with 70% ethanol and/or isopropanol solutions. The …
GFZ Helmholtz Centre for GeosciencesMolecular microbial ecology is crucial for understanding the role of microorganisms in ecosystems, as it provides insights into microbial diversity, interactions, and functions at the molecular level. Our labs utilize advanced techniques for environmental DNA and RNA isolation but also DNA sequencing, metagenomics, and enumeration of microbial cells to uncover the genetic and metabolic capabilities of microbes in all kinds of natural environments. This knowledge is vital for addressing global …
GFZ Helmholtz Centre for GeosciencesThe Omics Lab is currently equipped with all facilities to carry out DNA extractions, polymerase chain reactions (PCR) and gel electrophoresis. Current research includes the biodiversity and functions of cryophilic microbiomes with the focus on snow and ice algal communities.
GFZ Helmholtz Centre for GeosciencesProcesses related to the solvents extraction of snow and ice algae or particles are conducted in the Organic Microbial Geochemistry Lab. These processes are done in combination with ultrasonic bath, shaking, and solid phase extraction. Evaporation of organic extracts using nitrogen or argon gas, as well as various intermediate steps such as milling, acidification and filtration are also carried out in this lab. The subsequent analysis is done with the [Shimadzu Total Organic Carbon Analyzer …
GFZ Helmholtz Centre for GeosciencesOptical microscopes are available in the Microscopy Lab for different techniques, such as brightfield, polarization contrast, fluorescence. The microscopes can also be equipped with cameras
We also have the FlowCam 5000 by Yokogawa, which is a particle analyzer capable of taking optical images. It is comparable to a flow cytometer and features a syringe pump and 100 x 700 µm glass capillary, where liquid passes through. During a sample run, images of the flow are taken with a camera, …