The Cultivation Lab focusses on aerobic and anaerobic enrichment and isolation of bacteria and archaea (microbial cultivation) as well as morphological, physiological and phylogenetic characterization. It covers simulation experiments and investigates adaptation to extremes.

Studying the subsurface life also encounters the quantification of total cell abundance. Imaging and optical analysis methods are used for this. In the Flow Cytometry Lab, samples are studied via fluorescence microscopy for the visualization of microorganisms by fluorescent labelling. Thereby, phase-contrast images are used for direct observation of microorganisms. Flow cytometry is applied for cell detection and sorting.

Optical microscopes are available in the Microscope Lab for different techniques, such as brightfield, polarization contrast, fluorescence.

We also have the FlowCam 5000 by Yokogawa, which is a particle analyzer capable of taking optical images. It is comparable to a flow cytometer and features a syringe pump and 100 x 700 µm glass capillary, where liquid passes through. During a sample run, images of the flow are taken with a camera, magnified with a 10x objective. Detected particles are …