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- DNA Extraction
- DNA Isolation
- DNA Library Preparation
- DNA Sequencing
- Electrophoresis
- Environmental DNA
- FISH - Fluorescent In Situ Hybridization
- Fluorometry
- Gel Documentation
- Gel Electrophoresis
- Hybridization Assay
- NGS - Next Generation Sequencing
- PCR - Polymerase Chain Reaction
- qPCR - Quantitative …rase Chain Reaction
- Real-time PCR - Real…rase Chain Reaction
- Sanger Sequencing
- Shotgun Method
- UV-VIS Spectrophotometry
GFZ - German Research Centre for GeosciencesThe Molecular Microbiology Lab focuses on
I) DNA and RNA extraction, quantification and integrity applying protocols on commercial kits (physical and chemical cell lysis, silica column), phenol/chloroform extractions and extra- and intracellular separation (e/I DNA), and
II) microbiome diversity and abundance applying protocols on amplification and quantification of target genes via PCR and quantitative PCR, NGS library preparation as well as NGS sequencing.
Various sample types such …
GFZ - German Research Centre for GeosciencesThe Omics Lab is currently equipped with all facilities to carry out DNA extractions, polymerase chain reactions (PCR) and gel electrophoresis. Current research includes the biodiversity and functions of cryophilic microbiomes with the focus on snow and ice algal communities.
The main equipment consists of a low temperature chamber and a real-time thermal PCR Cycler. The environmental chamber (Percival LT-36VL) allows to perform incubation at set chosen environmental parameters, or following …
AWI - Alfred Wegener InstituteThe palaeogenetics lab is a part of the larger molecular genetic facility specialized to analyse ancient sedimentary DNA and is placed in a separate building apart from the modern and recent environmental lab and Post-PCR areas. In the palaeogenetics lab we analyse ancient DNA from sediment cores (lakes or marine archives) or permafrost deposits.
We apply DNA metabarcoding and metagenomics (shotgun and hybridisation capture) to investigate spatial and temporal biodiversity with the aim of …