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- DNA Extraction
- DNA Isolation
- DNA Library Preparation
- DNA Sequencing
- Electrophoresis
- FISH - Fluorescent In Situ Hybridization
- Fluorometry
- Gel Documentation
- Gel Electrophoresis
- NGS - Next Generation Sequencing
- PCR - Polymerase Chain Reaction
- qPCR - Quantitative …rase Chain Reaction
- Real-time PCR - Real…rase Chain Reaction
- UV-VIS Spectrophotometry
GFZ - German Research Centre for GeosciencesThe microbiology-related work of our section is done in this lab, as it has the capabilities for preparing a wide range of media and sterile solutions though filtration, autoclaving or UV exposure. Two refrigerators are dedicated for culturing cryophilic and cryotolerant microbial species under different light exposures. Moreover, a circulating water bath allows the culture of microorganisms up to 40 °C. The lab is also equipped with Bunsen burners, a PCR chamber and a Class II Biological …
GFZ - German Research Centre for GeosciencesThe Molecular Microbiology Lab focuses on
I) DNA and RNA extraction, quantification and integrity applying protocols on commercial kits (physical and chemical cell lysis, silica column), phenol/chloroform extractions and extra- and intracellular separation (e/I DNA), and
II) microbiome diversity and abundance applying protocols on amplification and quantification of target genes via PCR and quantitative PCR, NGS library preparation as well as NGS sequencing.
Various sample types such …
GFZ - German Research Centre for GeosciencesThe Omics Lab is currently equipped with all facilities to carry out DNA extractions, polymerase chain reactions (PCR) and gel electrophoresis. Current research includes the biodiversity and functions of cryophilic microbiomes with the focus on snow and ice algal communities.
The main equipment consists of a low temperature chamber and a real-time thermal PCR Cycler. The environmental chamber (Percival LT-36VL) allows to perform incubation at set chosen environmental parameters, or following …